Table 2.

Summary of mutation frequencies at targeted miRNA loci in T0 lines that express two sgRNAs.

Targeted rice pri-microRNA locus	Transgenetic CRISPR/Cas9 vector	Tested T0 seedling	Deletion T0 seedling mutant: number, ratio	Double NHEJ T0 seedling mutant: number, ratio	Single NHEJ T0 seedling mutant: number, ratio
OsMIR408	pZmUbi1::Cas9+OsU6::OsMIR408−sgRNA02+sgRNA04 (pZJP025)	20	12, 60.0%	8, 40.0%	0, 0.0%
OsMIR528	pZmUbi1::Cas9+OsU6::OsMIR528−sgRNA01+sgRNA03 (pZJP026)	22	5, 22.7%	13, 69.1%	1, 4.6%