Figure 1.

NRP1 promotes HH signaling at the level of GLI activation. A, HH-dependent luciferase reporter activity measured in NIH-3T3 cells transfected with the indicated constructs and stimulated with NSHH-conditioned medium (+NSHH). Data are reported as mean -fold induction ± S.D., with p values calculated using two-tailed Student's t test. n.s., not significant. B, top, Western blot analysis of NIH-3T3 lysates collected from cells expressing the indicated HA-tagged proteins. Bottom, quantitation of NRP levels relative to β-tubulin. a-β-Tub, anti-β-tubulin. C, schematic of various modes of HH pathway activation at the level of ligand (HH), small-molecule SMO agonist (SAG), oncogenic SMO mutations (SMOM2), and constitutive GLI activator (GLI2ΔN). D–F, luciferase reporter activity similar to A in NIH-3T3 cells stimulated with SAG (D) or co-transfected with SmoM2 (E) or GLI2ΔN (F). G and H, luciferase reporter activity similar to A in NIH-3T3 cells transfected with the Gli3 repressor.