Figure 3.

NRP1 TM dimerization is not required to promote HH signaling. A, left, schematic of full-length NRP1 and NRP1^G-V, in which three glycine residues are mutated to valines. Right, HH-dependent luciferase reporter activity measured in NIH-3T3 cells transfected with the indicated constructs and stimulated with NSHH-conditioned medium (+NSHH). Data are reported as mean -fold induction ± S.D., with p values calculated using two-tailed Student's t tests. n.s., not significant. B, left, schematic of NRP1^ΔECD and NRP1^ΔECD_G-V. Right, HH-dependent luciferase reporter activity measured in NIH-3T3 cells transfected with the indicated constructs and stimulated with NSHH-conditioned medium. Data are reported as mean -fold induction ± S.D., with p values calculated using two-tailed Student's t tests.