Figure 7.

TgPEPCK_mt regulates the flux of glutamine-carbon through gluconeogenesis and TCA cycle. A and B, violin plots showing the impairment of glutamine flux into metabolites involved in or directly associated with gluconeogenesis and TCA cycle in the Δtggt1/iΔtgpepck_mt mutant. Intracellular parasites were labeled as described in Fig. 6 (see “Materials and methods” for additional details). Fractional abundance of ¹³C in selected metabolites is depicted (n = 4 assays). The extent of variation in the contour of ATc-treated samples is proportional to the degree of perturbation in aggregate carbon flux via a given pathway.