Figure 2.

Blocking the proteasome restores Sec3-913 function at the restrictive temperature. A, wild-type and sec3-913 strains, along with strains where the endogenous sec3 promoter was replaced with the strong nmt1 promoter, were streaked on minimal medium as indicated (left panel) and incubated at 30 °C and 36 °C. B, the percentage of septated cells (septation index) was determined by calcofluor staining of the indicated strains with or without added 0.2 mm BZ as a function of incubation time at 36 °C (left panel). The percentage of septated cells with multiple septa after 4 h at 36 °C was determined by calcofluor staining with or without added 0.2 mm BZ (right panel). The error bars indicate the standard deviation (n = 4). *, p < 0.05; **, p < 0.01. C, calcofluor staining of sec3-913 cells at 30 °C and 36 °C with or without 0.2 mm BZ. Note that the thickness of the septa is reduced in the presence of BZ. Scale bar = 5 μm.