Figure 7.

Sec3-913 is a target of proteasome-associated deubiquitylating enzymes. A, growth on rich medium of the indicated mutant and double mutant strains was compared over a range of temperatures. Note that the temperature-sensitive growth defect of the sec3-913 single mutant is suppressed in sec3-913ubp3Δ cells and, to a lesser degree, in the sec3-913uch2Δ strain. B, calcofluor staining of sec3-913 and sec3-913ubp3Δ cells at 30 °C and 34 °C (left panel). Scale bar = 5 μm. The percentages of septated cells (septation index) and of septated cells with multiple septa were determined. The error bars indicate the standard deviation (n = 4). ***, p < 0.001. C, the steady-state level of Sec3-913 in the indicated strains at 29 °C and 36 °C was compared by SDS-PAGE and Western blotting using antibodies to V5 (to detect Sec3-913) and, as a loading control, to α-tubulin.