Figure 8.

Wild-type Sec3 levels are regulated by Pib1 and the proteasome. A, the steady-state level of wild-type Sec3 at 29 °C and 36 °C in cultures treated with BZ for 16 h was compared by SDS-PAGE and Western blotting using antibodies to V5 (to detect Sec3) and, as a loading control, to α-tubulin. Quantifications by densitometry are given below as -fold change ± standard error of the mean (n = 3). B, the steady-state level of wild-type Sec3 at 29 °C and 36 °C in either a WT or pib1Δ background was compared by SDS-PAGE and Western blotting using antibodies to V5 (to detect Sec3) and, as a loading control, to α-tubulin. Quantifications by densitometry are given below as -fold change ± standard error of the mean (n = 3). C, the steady-state level of wild-type Sec3 from cells grown at 29 °C (control) or cells subjected to a 45-min heat shock treatment at 40 °C was compared in the presence or absence of the proteasome inhibitor BZ or in a pib1Δ background by SDS-PAGE and Western blotting using antibodies to V5 (to detect Sec3) and, as a loading control, to α-tubulin. Quantifications by densitometry are given below as -fold change ± standard error of the mean (n = 3).