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. 2017 Jun 15;292(37):15254–15265. doi: 10.1074/jbc.M117.778530

Figure 3.

Figure 3.

SCCRO promotes Cul3 neddylation and is required for its localization to the midbody. A, Western blot analysis of cell lysates, showing that neddylation of Cul3 was reduced in SCCRO−/− MEFs more than other cullins compared with SCCRO+/+ MEFs. The asterisk denotes possible dineddylated Cul5. IB, immunoblot. B, Western blot analysis of cell lysates of infected SCCRO−/− MEFs, showing that retrovirus-mediated transfection with HA-SCCRO restored SCCRO expression to levels seen in SCCRO+/+ MEFs (center row, compare lanes 2 and 4) and rescued Cul3 neddylation (lane 4). EV = empty vector. C, immunostaining for cullins and Aurora B, showing that only Cul3 localizes to the midbody in SCCRO+/+ MEFs and also showing the absence of Cul3 in the midbody of SCCRO−/− MEFs and MLN4924-treated SCCRO+/+ MEFs. Note that anti-Cul4 did not work for immunostaining. Scale bar = 5 μm.