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. 2017 Jul 26;292(37):15312–15320. doi: 10.1074/jbc.M117.799346

Figure 2.

Figure 2.

The intracellular pool is composed of the CD33m isoform. A, CD33 exists in two splice forms: a full length CD33M and a truncated CD33m that is lacking the V-set domain. Human alleles that predispose individuals to Alzheimer's are spliced primarily into the CD33M form, alleles that protect from Alzheimer's are spliced primarily into CD33m. B, the left panel shows the schematic regarding the reactivity of different CD33 antibodies that can partially discriminate the two forms. WM53 targets the V-set domain and labels only CD33M (blue). HIM3-4 targets the C2set domain and labels both CD33M and CD33m (green). The middle panel shows the flow cytometry analysis of CD33M and CD33m+M using the antibodies WM53 and HIM3-4 with intact and permeabilized cells. The right panel shows the quantification of mean fluorescence intensity of CD33M and CD33m+M in intact and permeabilized cells. The line shows mean fluorescence intensity. **, p < 0.01, n = 4 for CD33m+M and n = 3 for CD33M.