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. 2017 Jul 26;292(37):15352–15368. doi: 10.1074/jbc.M116.736207

Figure 1.

Figure 1.

Effect of BMPs or pituitary CM on luciferase activity from C3H-B12 cells. A, dose-dependent induction of the BRE-Luc construct from C3H-B12 cells by BMPs. C3H-B12 cells were treated overnight with increasing concentrations of either BMP-2 (●), BMP-4 (■), BMP-6 (▴), or BMP-7 (ο) before assaying for luciferase activity as described under “Experimental Procedures.” Note that BMP-6 and BMP-7 curves are superimposed. Results are expressed as arbitrary units. Each point represents the mean ± S.E. of three experiments. B–D, effect of pituitary CM on luciferase activity from C3H-B12 cells. C3H-B12 cells were exposed overnight to DMEM or CM before assaying for luciferase activity. B, CM were from ovine pituitary cells treated or not with 10−8 m GnRH for 6 h (CM GnRH 6 h) or with 10−9 m activin for 48 h (CM activin 48 h). Values are the mean ± S.E. from seven experiments. C, pituitary media conditioned for 48 h (CM basal 48 h) were supplemented with increasing concentrations of BMP-4 before exposition to C3H-B12 cells. Values are the mean ± S.E. from three experiments. D, comparison of pituitary media conditioned for 6 h with 10−8 m GnRH (CM GnRH 6 h) or without GnRH (CM basal 6 h) or for 48 h with 10−9 m activin (CM activin 48 h) and supplemented with rh-BMP-4 (10 ng/ml) before exposition to C3H-B12 cells. Values are the mean ± S.E. from seven experiments. Inset, CM were from ovine adrenocortical cell cultures conditioned for 48 h (CM Basal 48) supplemented with BMP-4 (10 ng/ml) before exposition to C3H-B12 cells. Values are the mean ± S.E. from three experiments. E, effect of pituitary CM on C3H-B12 cell proliferation. C3H-B12 cells were exposed overnight to DMEM supplemented or not with BMP-4 (10 ng/ml), GnRH (10−8 m), or activin (10−9 m) or to pituitary CM (CM basal 6 h or CM basal 48 h) before assaying for cell proliferation. F, effect of pituitary media conditioned for 48 h (CM basal 48 h) and supplemented with BMP-2 (10 ng/ml) on luciferase activity from C3H-B12 cells. Values are the mean ± S.E. from three experiments. Bars with different letters indicate that group means are significantly different at p < 0.05.