Figure 7.

Poly(I:C) stimulation enhances the invasive ability of SW620 cells in a TLR3-dependent manner. A and B, SW620 and SW480 cells (A) or SW620 cells (B) transfected with silencing RNA against TLR3 (10 nm) or NS RNA were plated in a CytoSelect 96-well invasion plate and left untreated or stimulated with poly(I:C) (10 μg/ml) for 20 h. Cells that migrated through the membrane were lysed and quantified. Results were normalized to an untreated sample. C, SW620 treated with siRNA against TLR3 or NSRNA were assayed for TLR3 mRNA by qPCR to confirm gene silencing. -Fold change is shown relative to an untreated NS RNA sample. The results show mean ± S.D. of triplicates and are representative of three independent experiments. ***, p < 0.001; **, p < 0.01 versus medium or NS RNA treatment (two-way ANOVA, Bonferroni post-test).