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Phosphorylation of Shp2 at Tyr542 is regulated bidirectionally in synaptic scaling. A, hippocampal neurons were treated for 48 h with TTX or Bic, followed by Western blot analysis. MW, molecular weight. B, quantification of Ser(P)845, GluA1, Tyr(P)542, and Shp2 levels. Error bars indicate S.E. n = 4 independent experiments. **, p ≤ 0.01; #, p ≤ 0.001 against control; one-way ANOVA followed by Tukey's post hoc test.
