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. 2017 Aug 2;292(37):15481–15488. doi: 10.1074/jbc.M117.775239

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — Shp2 is accumulated at the postsynaptic site after TTX-mediated synaptic upscaling. A, hippocampal neurons were treated for 48 h with TTX, and colocalization of Shp2 (green) and PSD95 (red) was analyzed by immunocytochemistry. Scale bar = 5 μm. B, Manders coefficiency for colocalization of Shp2 and PSD95 and percentage of Shp2 colocalized with PSD95 (n = 22 or 18 neurons for the control condition or TTX treatment, respectively). #, p ≤ 0.001. C, hippocampal neurons were treated for 48 h with TTX, and the PSD fraction was analyzed. MW, molecular weight. D, quantification of GluA1 and Shp2 levels in the PSD fraction (n = 4 independent experiments). **, p ≤ 0.01; #, p ≤ 0.001 versus control.