Figure 2.

Effect of DRB on the distribution of RNA polymerase II on an early (UL39) and a late (UL38) gene. ChIP against RNA polymerase II was performed in 1BR.3.N cells infected with HSV-1 at 37 °C using an m.o.i. of 10. Virus was added to cells at 0 h, and cells were harvested at the indicated time points. Three independent experiments were performed, and for each experiment the individual observations were normalized using the UL39 promoter as reference. The mean value for each experimental point was calculated, and the error bars show the standard deviation. Experiments performed in the absence of DRB (a) and in the presence of 25 μm DRB (b).