Figure 6.

Effects of DRB on HSV-1 early and late transcripts, and DRB can still exert full inhibitory effect on a late gene expression added 7 h.p.i. a, ratios between UL38 and UL23 mRNAs were measured by multiplex RT-qPCR in cells infected with HSV-1 at an m.o.i. of 1. Then 25 μm DRB was added 1 h.p.i. in parallel with undisturbed infection. Cells were harvested at the indicated time. The mean values of three independent experiments are displayed, and the error bars indicate the standard deviation. b, expression of ICP8, glycoprotein C, and actin was measured by Western blotting at 37 °C at 19 h.p.i. at an m.o.i. of 1. The time of addition of 25 μm DRB to the culture medium is shown. c, HSV-1 transcriptome in cells infected with HSV-1 at an m.o.i. of 1 was analyzed at 13 h.p.i. by high throughput RNA sequencing. Sequencing was performed on infected cells in the absence of DRB (red) and in cells supplied with 25 μm DRB at 7 h.p.i. (blue). The results are expressed as ratio between reads mapping to a specific position relative to the total number of reads mapping to that gene.