Table 1.
Data collection and refinement
| PDB 5LC2 human monomer | PDB 5LC3 mouse monomer | PDB 5LC4 mouse dimer | |
|---|---|---|---|
| Data collection | |||
| Space group | P41 | C2 | P21 |
| Cell dimensions (Å) | 110.7 110.7 30.5 | 163.8 32.0 75.7, 112° | 47.8 118.4 85.4, 99° |
| Resolution (Å) | 39.1-1.59 (1.65) | 44.3-2.0 (2.1) | 48.5-1.84 (1.91) |
| Measured reflections | 240,458 (5580) | 77,440 (5,891) | 237,659 (21,793) |
| Unique reflections | 46,062 (2551) | 23,649 (1729) | 69,589 (6,480) |
| Rmerge | 0.058 (0.60) | 0.042 (0.46) | 0.078 (1.0) |
| 〈I/σI〉 | 14.4 (1.2) | 13.2 (2.1) | 8.2 (0.9) |
| Completeness (%) | 91.7 (53.4) | 98.9 (99.5) | 98.4 (92.7) |
| Redundancy | 5.2 (2.2) | 3.3 (3.4) | 3.4 (3.4) |
| Refinement | |||
| Resolution (Å) | 30.0-1.80 (1.85) | 44.2-2.0 (2.13) | 48.5-1.84 (1.89) |
| Rwork/Rfree | 0.17/0.21 | 0.22/0.24 | 0.21/0.23 |
| No. atoms | |||
| Protein | 2,592 | 2,499 | 5,209 |
| Water | 447 | 105 | 423 |
| Average B-factors | |||
| Protein (Å2) | 25.3 | 61.7 | 39.0 |
| Water (Å2) | 37.2 | 51.1 | 46.3 |
| Ramachandran outliers (%) | 0.6 | 1.3 | 0.8 |
| Root mean square deviations | |||
| Bond lengths (Å) | 0.010 | 0.010 | 0.008 |
| Bond angles (°) | 1.06 | 1.20 | 1.00 |