Figure 6.

PKM1 or PKM2 silencing differentially affects the viability of H1299 and A549 (human lung cancer cells). A and B, CCK8 assay to examine the viability of H1299 cells (A) and A549 cells (B), transduced with vector (pLKO.1), shPKM1, or shPKM2 and cultured for the period of 72 h, the cellular viability assayed for every 24 h; with statistical analysis (where n ≥ 3; mean ± S.D.), and the level of significance was tested using two-way analysis of variance with Tukey's multiple comparisons test. C and D, bars represent the number of colonies obtained from the anchorage-dependent clonogenic assay of H1299 (C) and A549 (D) stable cells transduced with lentivirus containing empty vector (pLKO.1), shPKM1, or shPKM2; with statistical analysis (where n ≥ 3; mean ± S.D.), and the level of significance was tested using unpaired Student's t test.*, p < 0.01.