Figure 3.

Effect of RACE-SEQ Data Processing Using a Proprietary High Computational Overhead Pipeline versus the Newer Pipeline Presented Herein for Three Separate siRNAs

(A–C) siRNA6 (A), siRNA19 (B), and siRNA22 (C) target independent sites on the HCV replicon genome. Driving Slicer-mediated cleavage of the HCV replicon RNA genome suggests less than 0.001% variance to maximal incidence counts. Changes in novel 5′ read counts (y axis) at each position on the HCV genome targeted by an siRNA (x axis; HCV genome in a 5′ to 3′ fashion) is represented in log10 scale reflecting the data distribution range for 5′ end incidence counts.