Fig. 3.

NO levels in W303 andΔyhb1strains after menadione treatment. Yeast cells were grown in YPG and, where indicated, menadione was added to the culture media. A, NO levels were measured using DAF-FM DA and flow cytometry. Data are represented as means±SD from 3 independent experiments. * and ** indicate significant (p < 0.05) or highly significant (p < 0.01) differences, respectively, when compared to control condition.; B, the presence of nitrotyrosines was evaluated in crude extracts by western blot using antibodies which recognize this prostraductional modification.