Fig. 5.

Activation of Aft1 by iron scarcity is no prevented inΔyhb1cells. A, the indicated strains were grown in YPD media with or without 100 μM BPS and the localization of Aft1-GFP analyzed by fluorescence microscopy. B, histograms show the percentage of cells showing nuclear localization of Aft1-GFP in each indicated condition. Data are represented as means ± SD from 3 independent experiments in which at least 100 cells were analyzed. * and ** indicate significant (p < 0.05) or highly significant (p < 0.01) differences, respectively, when compared to control condition. C, the indicated strains were grown in YPG media with or without BPS and FET3 mRNA expression was measured by qPCR. Data are represented as means ± SD from 3 independent experiments. D, the indicated strains were grown in YPD to OD600 = 0.5 and serial dilutions (1:5) where plated on YPD plates supplemented or not with BPS.