Fig. 6.

Activation of Aft1 by Grx5 deficiency is not prevented by NO. Yeast cells were grown in YPG and, where indicated, doxycycline was added to the culture media for 24 h in order to repress Grx5 expression. Whole cell iron content was measured by the BPS method as indicated in experimental procedures. Data are represented as means±SD from 3 independent experiments. * and ** indicate significant (p < 0.05) or highly significant (p < 0.01) differences, respectively, when compared to control condition.