Fig. 1.
Device structure and working mechanism. (a) Schematic drawing illustrating the working principle: (i) mechanical disruption of the cell membrane as the cell passes through the constriction, and (ii) he subsequent electric pulses driving DNA into the cytoplasm and nucleus through the disrupted membrane. (b) Magnification of a set of identical parallel microfluidic constriction etched into a silicon wafer (left), and a set of electrodes deposited on a Pyrex wafer (right). (c) An optical image of a finished device realized by bonding silicon and Pyrex wafers. The scale bar is 1 mm. Details of device fabrication are in Figure S1.