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. Author manuscript; available in PMC: 2017 Sep 18.
Published in final edited form as: Nat Biomed Eng. 2017 Mar 9;1:0039. doi: 10.1038/s41551-017-0039

Fig. 4.

Fig. 4

ESCRT-III recruitment for plasma membrane and nuclear envelope repair. CHMP4B-GFP expressing Hela cells were stained with DAPI to visualize the nucleus before treatment. After treatments of squeezing (S), EP, or DFE, cells were incubated in culture media, and fixed with a cell fixation kit at 0.5, 1.5, 2.5, 3.5, 5.5, 10, and 15 minutes after treatment, and processed for confocal imaging. (a) Confocal images of representative cells at 1.5 minutes after no treatment (NC, Negative Control), squeezing (S), electroporation (EP), and DFE. CHMP4B-GFP foci are visible at both plasma membrane and nuclear envelope in DFE, but only at the plasma membrane in S and EP. Number of CHMP4B-GFP foci at plasma membrane (b) and nuclear envelope (c) after treatment of S, EP and DFE (10 cells at each data point). Original confocal fluorescent images are shown. Each data point represents the mean value of 10 cells and error bars represent ± s.d..