Figure 2.

Cell Uptake Summary

Uptake of ISIS 304801, ISIS 396443, ISIS 420915, and ISIS 681257 in human hepatocytes. (A and B) Concentration ratio in pellet normalized to supernatant amount at time 0 (A) and percent of supernatant normalized to time 0 (B). Incubation amount refers to μM for ISIS 304801, 420915, and 681257 and μg/mL for ISIS 396443, respectively. Samples, curves, QCs, and internal standard were processed by liquid-liquid extraction and solid phase extraction prior to analysis by LC-UV/MS. Two calibration curves were used, with the linear range for LC-MS and LC-UV/Vis as 0.064–641.03 μM and 32.05–4,807.69 μM, respectively.