Figure 4. Overview of the liposome disruption assay.

The cuvette with buffer has no fluorescence. Addition of liposomes results in a minor increase in fluorescence due to residual free carboxyfluorescein outside the liposomes. Addition of lytic protein or biomolecule disrupts liposomes resulting in the release of carboxyfluorescein and subsequent dequenching of fluorescence. Finally, addition of Triton-X disrupts all the liposomes in the cuvette.