Fig 6. Blood stage growth of berghepain-1 knockout parasites in phenylhydrazine (PHZ)-treated mice.

Swiss Webster mice were pre-treated with PHZ (squares) or PBS (circles) with the last dose administered 3 days prior to i.v. injection of 2x10⁶ erythrocytes infected with BP1-CON or BP1-KO parasites. Pooled results from three biological replicates are shown, two using BP1-KO clone 1 and one using BP1-KO clone 2. A. Parasitemia was monitored daily and the average parasitemia of all surviving mice is plotted. B. The graph displays the data from days 1 through 8 (boxed region of panel A), to highlight differences in parasitemia in the early stage of infection. C. Survival curves of PHZ- and PBS-treated mice after infection with BP1-CON and BP1-KO parasites. Mice were monitored for 30 days and the average survival rate of infected mice is plotted.