Table 2. Liver and blood stage merozoite infectivity of berghepain-1 knockout parasites.

Swiss Webster mice were injected i.v. with purified blood stage schizonts or merosomes of BP1-CON and BP1-KO clone 1 parasites and parasitemia was monitored daily. In the last set of experiments, mice were pre-treated with phenylhydrazine (PHZ) prior to inoculation of BP1-CON and BP1-KO merosomes.

Parasite	Dose	Infectivity (#mice infected #mice inoculated)	Prepatent period (days)
BP1-CON	1,000 schizonts	5/5	4.00
BP1-KO	1,000 schizonts	5/5	4.60
BP1-CON	10,000 schizonts	5/5	3.00
BP1-KO	10,000 schizonts	5/5	4.00
BP1-CON	1,000 merosomes	20/20	1.00
BP1-KO	1,000 merosomes	20/20	5.65
BP1-CON	1,000 merosomes	10/10 PHZ-treated mice	1.00
BP1-KO	1,000 merosomes	10/10 PHZ-treated mice	4