Table 2.
Overview of heterologously expressed bacterial laccase (origin, host, gene size, molecular weight, fermentation conditions, optimum temperature and stability, optimum pH and stability, family, etc.)
| S. no. | Origin | Host | Gene size (bp)/enzyme (aa/kDa) | Substrate | Carbon source/fermentation conditions | Temp. optima (°C) of activity | Temp. stability | pH optima of activity | pH stability | Reference |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Bacillus vallismortis fmb-103 |
Escherichia coli
BL21 |
1542 bp/513 aa/70 kDa | ABTS | LB/16 °C/24 h | 84 | 50%/70 °C/pH 4.8/10 h | 4.8 | 90%/pH 8.0/45 °C/10 d | Sun et al. (2017) |
| 2 | Bacillus subtilis |
Escherichia coli
DH5α |
NR | ABTS | NR | 30 | NR | 11.6 | NR | Wang and Zhao (2016) |
| 3 | Meiothermusruber DSM 1279 | E. coli DH5α | 1497 bp/499 aa/50 kDa | ABTS | LB/30 °C/180 rpm | 70 | 50%/60C/120 min | 5.0 | NR | Kalyani et al. (2016) |
| 4 | PaenibacillusglucanolyticusSLM1 |
Escherichia coli
BL21 (λDE3) |
1701 bp/567 aa/90 kDa | ABTS | LB/37 °C/200 rpm/6 h | 40 | 76%/60 °C/pH 7.0/4 h | 7.0 | 68%/pH 5-7/24 h | Mathews et al. (2016) |
| 5 | B. subtilis | E. coli | 62 kDa | ABTS | NA/37 °C/48 h/pH 7.0 | NR | NR | 8.0 | NR | Menaka et al. (2015) |
| 6 | Bacillus coagulans LMCO | E. coli | 1566 bp/522 aa/59.7 kDa | ABTS | LB/30 °C/180 rpm | 30 | >80%/70°C/30 min | 4.0 | NR | Ihssen et al. 2015 |
| DMP | 7.5 | |||||||||
| SGZ | 7.5 | |||||||||
| Guaiacol | 8.5 | |||||||||
| 7 |
Thermus
thermophilus SG0.5JP17-16 |
Pichia pastoris | 1398 bp/466 aa/46 kDa | ABTS | LB/37 °C/200 rpm | 90 | >75%/80°C/4 h | 4.5 | >95%/pH 4-11/30°C/12 h | Liu et al. (2015) |
| SGZ | 6.0 | |||||||||
| Guaiacol | 7.5 | |||||||||
| DMP | 8.0 | |||||||||
| 8 | P. ananatis | E. coli | 120 kDa | ABTS | LB/30 °C/170 rpm/6 d | 30–50 | >40%/60 °C/10 min/pH 2.5 | 2.5 | 100%/pH 3.0/4 °C/12 h | Shi et al. (2015a, b) |
| Guaiacol | 4.5 | |||||||||
| 9 | Bacillus subtilis | E. coli | 1542 bp/513 aa/62 kDa | SGZ | LB/37 °C/220 rpm/20 h | 37 | NR | 5.0 | NR | Ghasemi et al. (2014) |
| 10 | B. pumilus strain W3 | E. coli | NR | ABTS | LB/37 °C/48 h | 50 | NR | 3.0–9.0 | 100%/pH 7.0–9.0/50 °C/10 d | Guan et al. 2014 |
| SGZ | ||||||||||
| DMP | ||||||||||
| 11 | B. licheniformis | Pichia pastoris | 1542 bp/513 aa/59.07 kDa | SGZ | LB/37 °C/200 rpm/12 h | 70 | 50%/70/6.9 h | 6.2 | 100%/7–9/30 °C/10 days | Lu et al. 2013 |
| 12 | Uncultured Bacterium lac21 | E. coli | 1389 bp/463 aa/52 kDa. | SGZ | NR | 45 | >60%/35 °C–50 °C | 7.5 | 95%/pH 6.0–8.0/4 °C/1 h | Fang et al. (2012) |
| 13 | Ochrobactrum.sp.531 | E. coli | 1092 bp/364 aa/57.8 kDa | DMP | LB/23 °C | 37 | NR | 8.0 | >80%/pH 7.5/37 °C | Li et al. (2012) |
| ABTS | 3.6 | |||||||||
| SGZ | 7.5 | |||||||||
| 14 | B. pumilus DSM 27 | E. coli | 1530 bp/510 aa/58.6 kDa | ABTS | SRB/30 °C/150 rpm/24 h | 37 | NR | 4.0 | NR | Reiss et al. (2013) |
| DMP | 7.0 | |||||||||
| SGZ | 6.5 | |||||||||
| 15 | B. licheniformis | E. coli | 1069 bp/513 aa | ABTS | TB/37 °C/600 rpm/24 h | 37 | NR | 4.0 | 100%/p H 5.0–7.0/37–70 °C | Koschorreck et al. (2009) |
| 16 | Aeromonashydrophila WL-11 | Escherichia coli | 1605 bp/534 aa/58.5 kDa | ABTS | LB/20 °C/200 rpm | 37 | 40%/70 °C/10 min | 2.6 | NR | Wu et al. (2010) |
| DMP | 8.0 | |||||||||
| 17 | Streptomyces ipomoea CECT 3341 | E. coli | 1008 bp/336 aa | ABTS | YEME medium/28 °C/180 rpm | 37 | NR | 5.0 | NR | Guijarro et al. (2009) |
| DMP | 8.0 | |||||||||
| 18 | B. subtilis | E. coli | 1069 bp/513 aa/65 kDa | ABTS | LB/30 °C | 75 | 50%/80 °C/112 min | 3.0 | NR | Pereira et al. (2008) |
| SGZ | 7.0 |
LB Luria–Bertani, NA nutrient agar, SRB sulfate reducing bacteria medium, TB Terrific Broth, YEME yeast extract malt extract medium, DMP 2,4 dimethoxy phenol, ABTS 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, SGZ syringaldazine