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. 2017 Sep 11;10:4465–4474. doi: 10.2147/OTT.S143612

Figure 3.

Figure 3

miR-20a targets EGR2 3′-UTR.

Notes: (A) Sequence of mature miR-20a and its predicted target sequence of EGR2 3′-UTR and positions of mutation (EGR2 3′-UTR-mut). (B) EGR2 expression of U266 cells transfected with miR-20a mimics or inhibitor determined by Western blot. β-actin was used as the loading control. (C) Dual-luciferase assay of U266 cells co-transfected with the WT (or mut) EGR2 3′-UTR-psiCHECK2 reporter vector and miR-20a mimics or miR-20a-in. *p<0.05.

Abbreviations: miR-20a, microRNA-20a; WT, wild-type; EGR2, early growth response protein 2; 3′-UTR, 3′ untranslated region; mut, mutated; NC, negative control; miR, microRNA; in, inhibitor.