Fig. 1.

Flowchart of the image processing approach. Green-channel images and red-channel images are input into nucleus segmentation. Two-channel images are fused together for further thresholding segmentation, seed-based segmentation and precise segmentation. Next, several features are extracted from the segmented nucleus and are filtered by feature selection. Then, the selected features are applied for classification. Finally, the classified images are quantified for morphological analysis