Fig. 6.

Precise segmentation process. a The precise segmentation pipeline. i is the roughly segmented nucleus on the fused image. ii is the nucleus extracted from i. iii is a pure intensity background we constructed, whose gray value is the mean intensity of the boundary (the white line in i). iv is the image combined by ii and iii. v shows the new nuclear boundary. vi is the extracted nucleus. vii is the original background in fused image. viii is the final result of precise segmentation. b The result of k-means clustering. The x-axis is I and the y-axis is B. The blue circles represent the background pixels and the red ones represent the foreground pixels. The blue circle that the red arrow points to denotes all the pixels in iii. These pixels have the same I and B values