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. 2017 Jun 27;31(10):4472–4481. doi: 10.1096/fj.201700091R

Figure 5.

Figure 5.

FOXD3-AS1 functions as a miRNA sponge for miR-150. A) The position of miR-150 regulatory element in FOXD3-AS1 is shown. Mutation (underlined) was introduced into FOXD3-AS1 to disrupt base-pairing with miR-150 seed sequence. B) Schematic diagram of the strategy for constructing luciferase report plasmids. The WT and Mut forms of FOXD3-AS1 were cloned into the pRL-TK plasmid downstream of the luciferase gene. C) Mimic control or miR-150 mimic was cotransfected with FOXD3-AS1 WT or Mut reporter into Beas2B cells. pGL3 luciferase plasmid was also cotransfected for normalization. D) Beas2B and A549 cells were transfected with scramble or ASO, followed by 2 and 3 d hyperoxia exposure, respectively. The relative level of miR-150 was measured using real-time PCR. E) Beas2B and A549 cells were transfected with mimic control or miR-150 mimic, followed by 2 and 3 d hyperoxia exposure, respectively. The relative expression level of FOXD3-AS1 was measured using real-time PCR. Data represent 3 independent experiments. *P < 0.05, **P < 0.01.