Figure 6.

MiR-150 is negatively regulated by oxidative stress or hyperoxia. A–C) A549, Beas2B, and primary lung epithelial cells were exposed to the hyperoxia for the indicated times. The expressions of miR-150 in A549 (A), Beas2B (B), and primary lung epithelial (C) cells were determined using real-time PCR. D) A549 and Beas2B cells were treated with 90 µM H₂O₂ for 6 h. The expression level of miR-150 was measured using real-time PCR. Data represent 3 independent experiments. *P < 0.05, **P < 0.01.