Figure 2.

RORα binds to the promoter region of Sema3e. A) Positions of Rora DNA binding elements (i.e., ROREs), with the consensus sequence GGTCA, on the promoters of mouse Sema3d and Sema3e genes. B) ChIP assays were performed with P17 WT OIR retinas, and DNA fragments bound to immunoprecipitated RORα were quantified with qPCR with primers flanking potential RORE sites. RORE site 1 of Sema3e showed the strongest enrichment. None of the other RORE sites in Sema3e and Sema3d analyzed showed significant enrichment compared with IgG control. Data were normalized to IgG control. Primers of Opn1mw was used a positive control, and Hbb was as a negative control (n = 3/group). Data are presented as means ± sem. *P < 0.05, ***P < 0.001.