Figure 4.

GOT knockdown attenuates the neuroprotection of BCA. A–D) Transfection with GOT siRNA decreased GOT mRNA expression (A), protein expression (B, C), and enzyme activity (D). Transfected cells were treated with BCA (24 h), then challenged with glutamate for 24 h. Cells with GOT knockdown were more susceptible to glutamate-induced toxicity. *P < 0.05. E, F) Cell viability was measured after 24 h of glutamate challenge by LDH leakage (E) or calcein-AM (green, live cells) and propidium iodide (PI; red, dead cells) staining (F). Original magnification, ×10. GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Results are presented as means ± sd. *P < 0.05 for effect of glutamate; ^†P < 0.05 for effect of BCA; ^#P < 0.05 for effect of siRNA.