Figure 6.
Overexpression of APP increases the strength and duration of α2AAR-mediated ERK signaling. HEK293 cells were cotransfected with cDNA vectors encoding HA-α2AAR together with APP or empty vector. A) Representative Western blots showing p- and t-ERK in HEK293 cells, with or without APP overexpression. B) Quantification of ERK2 activation with 10 µM NE (plus 1 µM prazosin and 1 µM propranolol) treatment for 5 min. Data (means ± sem) are expressed as fold change vs. control cells expressing empty vector (defined as 1.0). C) Quantification of the relative change in ERK1/2 activation at 10 vs. 5 min of NE treatment. *P < 0.05, **P < 0.01 (Student’s t test; n = 8 per group).