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. 2017 Sep 15;3:17. doi: 10.1186/s40851-017-0078-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Mapping of Halocynthia roretzi Hox genes onto metaphase chromosomes (a-d). Metaphase chromosome spreads were prepared from cleavage stage Hr embryos and hybridized with two or three probes labeled with digoxigenin (red) or biotin (green) for genes indicated at the top of each panel. Chromosomes were stained with DAPI. Red and green arrowheads indicate signals for the gene of the same color code. In the right bottom corner of each panel, enlargement of one of the chromosomes with signals is shown in inset. Within a chromosome, a pale blue stained region corresponds to the centromeric region. The bar in d indicates 5 μm and is applicable to all panels. BAC clones used for probes were 5 J1 (Hox1), 3C14 (Hox2, Hox3 and Hox4), 6B23 (Hox5 and HoxX) and 1 J20 (Hox10, Hox11/12/13.a and Hox11/12/13.b)