Fig. 1.

Generation of BMDCs. Porcine BMDCs were cultured in RPMI 1640 medium supplemented with GM-CSF and IL-4. a The clusters of BMDCs on day 5, as viewed by optical microscopy at 100 ×magnification. b Morphology of cells after 6 days of culture, dendritic processes could be observed using optical microscopy at 400 × magnification. c Porcine BMDCs were cultured in RPMI 1640 medium supplemented without GM-CSF and IL-4 as control