Fig. 3.

Cytokines mRNA expression levels of DCs after vaccine Mhp-168 exposure. a IL-10; b IL-12; c IFN-γ. BMDCs were treated with vaccine Mhp-168 or LPS (10 ng/mL) for 8 h, 12 h and 24 h. The mRNA expression levels of IL-10, IL-12, IFN-γ were detected by real-time quantitative PCR (RT-qPCR). Data are expressed as means ± SD (n = 3 per group). All experiments were performed independently three times. Statistical significance was assessed by Student’s t-test. Differences were considered significant at (*) 0.01 < p < 0.05, (**) p < 0.01