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. 2017 Sep 15;19:11. doi: 10.1186/s12575-017-0060-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Affinity of the original antibody is retained even when additional binding sites are added. The affinity of the original antibody (in green) to its antigen was measured for a number of multivalent formats with ELISA