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. Author manuscript; available in PMC: 2017 Sep 19.
Published in final edited form as: Mol Cell. 2012 Oct 4;48(4):601–611. doi: 10.1016/j.molcel.2012.08.029

Figure 2. p21 and denatured (molten-globule) ovalbumin are degraded by mammalian 20S and 26S proteasomes with either terminus blocked.

Figure 2

Time course of p21 N or C terminally fused to RFP degradation by mammalian 20S proteasome (A) or 26S proteasome (B). (C) Large excess of p21-RFP & RFP-21 (3µM) were incubated with 26S proteasome (1nM), a portion (1:5) of the initial material and following one hour of incubation was blotted with anti RFP. (D) Time course of ova-RFP and RFP-ova degradation by mammalian 26S proteasomes. Right: Quantification of the residual amount of protein at each time point, are presented (SD were calculated from four repetitions under similar conditions).