. 2017 Aug 30;2017:5076029. doi: 10.1155/2017/5076029

Table 4.

Anticholinesterases activity.

	Extract	% inhibition					µg/mL
	Extract	Concentration (µg/mL)					µg/mL
		50	100	200	400	800	IC₅₀

Acetylcholinesterase inhibitory activity	Ethanol bark	10.46 ± 2.13	28.68 ± 1.97	30.11 ± 2.37	36.90 ± 2.20	52.13 ± 0.48	804.88 ± 48.69^b
	DCM : M bark	19.77 ± 2.16	32.34 ± 0.61	37.88 ± 0.11	40.26 ± 0.99	47.69 ± 1.05	966.68 ± 63.18^a
	Ethanol leaf	6.84 ± 1.44	13.44 ± 2.52	30.08 ± 0.08	35.04 ± 1.59	46.33 ± 3.75	810.96 ± 79.98^a
	DCM : M leaf	−10.66 ± 3.65	4.03 ± 1.37	14.93 ± 2.31	34.34 ± 1.58	49.13 ± 0.63	879.35 ± 68.00^a

	Bark	6.25	12.5	25	50	100	IC₅₀

Butyrylcholinesterase inhibitory activity	Ethanol	8.50 ± 2.94	17.17 ± 3.69	42.14 ± 3.01	66.06 ± 1.19	75.10 ± 0.75	36.09 ± 0.83^c
Butyrylcholinesterase inhibitory activity	DCM : M	9.06 ± 2.98	36.79 ± 2.69	50.60 ± 4.42	67.16 ± 0.92	78.97 ± 1.68	26.62 ± 1.66^d

	Leaf	25	50	100	200	400	IC₅₀

Butyrylcholinesterase inhibitory activity	Ethanol	5.42 ± 4.07	25.84 ± 2.20	35.28 ± 1.14	42.62 ± 2.66	49.22 ± 1.81	340.60 ± 18.23^a
Butyrylcholinesterase inhibitory activity	DCM : M	6.57 ± 2.12	22.95 ± 2.03	37.45 ± 0.38	42.28 ± 0.63	57.48 ± 1.81	261.96 ± 19.56^b

Data represented as mean ± SEM (n = 4 each). Mean IC₅₀ values in the column superscripted by different letters are significantly different at p < 0.05. Statistical analysis was carried out separately for acetylcholine esterase and butyrylcholine esterase inhibitory assays. IC₅₀ galantamine, acetylcholine esterase inhibitory activity: 2.52 ± 0.17 µg/mL; IC₅₀ galantamine, butyrylcholine esterase inhibitory activity: 74.80 ± 3.53 µg/mL. Ethanol bark, DCM : M bark, ethanol leaf, and DCM : M leaf r² = 0.97, 0.97, 0.94, and 0.98, respectively, for butyrylcholine esterase inhibitory activity. Ethanol bark, DCM : M bark, ethanol leaf, and DCM : M leaf r² = 0.92, 0.94, 0.95, and 0.99, respectively, for acetylcholine esterase inhibitory activity. DCM : M: dichloromethane : methanol.