Embryos derived from zic2agbt133/+; zic2bt104/zic2bt104 parents were stained for alx1 expression by WISH. A: normal expression in periocular mesenchyme of sibling embryo. B: depleted expression in zic2 (arrow) in mutant embryo (39 out of 112 total, 2 expts). C–G: wild type embryos stained for alx1 expression by WISH. C–D: alx1 is expressed in frontonasal neural crest at 16 hpf. E, F: alx1 is expressed in periocular mesenchyme (*) at 24 hpf and 36 hpf. G: alx1 is expressed in the ethmoid plate (arrowhead) at 48 hpf. Embryos in A, B, C, and E are shown in lateral views, anterior to the left. Embryo in D is shown dorsally, anterior to the left. Embryos in F and G are shown in anterior views, ventral at the top.