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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biomaterials. 2017 Jul 10;142:1–12. doi: 10.1016/j.biomaterials.2017.07.011

Figure 1. Characterization of Mϕ exosomes.

Figure 1

(a) Number-weighted size distribution of exosomes by NTA (different colors show three repeated measurements). (b) Western blotting of RAW Mϕs and exosome lysates at comparable protein loading amounts showing exosomal markers and conserved proteins. (c) Concentration-dependent uptake of CM-DiI labeled exosomes at 4 h. (d) Inhibition of uptake of CM-DiI labeled exosomes (0.6×1010 exosomes/ml at 4 h) by non-labeled exosomes. *** p < 0.001 vs untreated cells background (bkg) or indicated groups. Exosomes were purified by sequential centrifugation of RAW Mϕs-conditioned medium. Cell uptake was determined by Flow cytometry. Data are mean fluorescence intensity (MFI) ± SD of 5000–10000 live singlets, n = 3, * p < 0.05 and *** p < 0.001 vs. untreated cells or indicated groups by one-way ANOVA and post Newman-Keuls multiple comparison test.