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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biomaterials. 2017 Jul 10;142:1–12. doi: 10.1016/j.biomaterials.2017.07.011

Figure 2. ICAM-1/LFA-1 mediate uptake of Mϕ exosomes in hCMEC/D3 cells.

Figure 2

(a) Expression of LFA-1 in Raw Mϕs and Mϕ-derived exosomes by western blotting at equal protein loading. (b) Expression of ICAM-1 in hCMEC/D3 cells in response to 3 or 6 h of stimulation with LPS (100 ng/ml). (c) Uptake of exosomes in hCMEC/D3 cells with or without 3 or 6 h of LPS stimulation. (d) Effect of co-incubation with anti-ICAM-1 or anti-LFA-1 antibodies (100 µg/ml) on cell uptake of exosomes in hCMEC/D3 cells. Cell uptake was determined by flow cytometry after 4 h incubation with CM-DiI labeled exosomes (0.6×1010 exosomes/ml). Data are MFI ± SD of 5000–10000 live singlets, n = 3, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. indicated groups by one-way ANOVA and post Newman-Keuls multiple comparison test.