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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: J Immunol Methods. 2017 Aug 2;450:66–72. doi: 10.1016/j.jim.2017.07.009

Figure 3. Fluorophore-conjugated antibodies have consistent distribution between Cy5 and Cy3 fluorophores.

Figure 3

Fluostar fluorescent intensity measurements for Gamunex-C Cy5(red) and Gamunex-C Cy3(blue) over time for plasma, cervico-vaginal mucus, and vaginal-weck cel fluid with spearman correlation inset (A). Temporal analyses of mean fluorescent intensities of Gamunex-C Cy5 (red) and Gamunex-C Cy3 (blue) in macaque vaginal biopsies (left) and rectal biopsies (right) (B) each displayed dot represents individual images analyzed. Fluorescent deconvolution images (40×) of infused Cy5 and Cy3 labeled Gamunex-C in macaque vaginal tissue(C). Top: Gamunex-C Cy5 (red), E-cadherin (green), and DAPI (blue) Middle: Gamunex-C Cy3 (blue), E-cadherin (green) Bottom: Gamunex-C Cy5 (red), Gamunex-C Cy3 (blue), E-cadherin (green). Size bars are 40 µm.