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. Author manuscript; available in PMC: 2017 Sep 19.
Published in final edited form as: Cell. 2011 Jun 10;145(6):875–889. doi: 10.1016/j.cell.2011.05.017

Figure 6. Oct4 specifically represses the NE lineage and Sox2 the ME lineage.

Figure 6

(A) Scatter plot of Oct4 vs. Sox1 in 2500 cells from a population in which constitutive Oct4 plasmid has been transfected and neural differentiation inducing signal retinoic acid (1μM) was added. Oct4 levels are measured as fold change over levels in ES cells under pluripotency conditions (B) Images of cells from A co-stained for Oct4, Sox2, Sox1, and DAPI. (C) Images of cells transfected with a constitutive Oct4 plasmid for 24 hours prior to CHIR induced ME differentiation co-stained for Oct4, Brachyury, and DAPI. (D) Scatter plot of Brachyury vs. Sox2 in over 5000 cells transfected with a Sox2 plasmid 24 hours prior to the induction of mesendodermal differentiation with 200ng/ml Wnt3a (identical results obtained with CHIR, data not shown) co-stained for Sox2, Brachyury and DAPI (E) Images of cells from D (F) Images of cells transfected with siRNA against Oct4 and a constitutive Sox2 plasmid co-stained for Oct4, Sox2, Brachyury and DAPI. (G) Images of ES cells transfected with a Brn2 plasmid and stained for Brn2 and Oct4 under pluripotency promoting conditions. See also Figure S5.