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. 2017 Apr 10;2:10. doi: 10.1038/s41541-017-0011-y

Table 1.

Binding capacity of sera from Pf rCSP-CAF09-immunized mice to P. falciparum and P.b.-P.f. CSP-FL CD8CT transgenic sporozoites

Immunization Qualitative score of tested sera—P. falciparum sporozoites
1:1000 1:4000 1:16000 1:64000 1:256000 1:1024000
Pf rCSP-CAF09 2× +++ +++ ++ ++ +
Pf rCSP-CAF09 3× +++ +++ +++ ++ + +/−
CAF09 3×
Immunization Qualitative score of tested sera—P.b.-P.f. CSP-FL CD8CT sporozoites
1:1000 1:4000 1:16000 1:64000 1:256000 1:1024000
Pf rCSP-CAF09 2× +++ +++ ++ ++ +
Pf rCSP-CAF09 3× +++ +++ ++ ++ + +/−
CAF09 3×

Pooled serum samples from mice immunized two or three times (n = 6/group) with Pf rCSP-CAF09 were incubated on slides coated with fixed sporozoites and stained with fluorescently labeled anti-mouse secondary antibody. Slides were qualitatively scored under a microscope

+++, strong;++, good;+, weak; and − no reactivity

Pf rCSP-CAF09, P. falciparum recombinant circumsporozoite protein in adjuvant CAF09; P.b.-P.f. CSP-FL CD8CT, P. berghei–P. falciparum CSP full-length CD8 epitope C-terminus transgenic parasite