Table 1.
Immunization | Qualitative score of tested sera—P. falciparum sporozoites | |||||
---|---|---|---|---|---|---|
1:1000 | 1:4000 | 1:16000 | 1:64000 | 1:256000 | 1:1024000 | |
Pf rCSP-CAF09 2× | +++ | +++ | ++ | ++ | + | − |
Pf rCSP-CAF09 3× | +++ | +++ | +++ | ++ | + | +/− |
CAF09 3× | − | − | − | − | − | − |
Immunization | Qualitative score of tested sera—P.b.-P.f. CSP-FL CD8CT sporozoites | |||||
---|---|---|---|---|---|---|
1:1000 | 1:4000 | 1:16000 | 1:64000 | 1:256000 | 1:1024000 | |
Pf rCSP-CAF09 2× | +++ | +++ | ++ | ++ | + | − |
Pf rCSP-CAF09 3× | +++ | +++ | ++ | ++ | + | +/− |
CAF09 3× | − | − | − | − | − | − |
Pooled serum samples from mice immunized two or three times (n = 6/group) with Pf rCSP-CAF09 were incubated on slides coated with fixed sporozoites and stained with fluorescently labeled anti-mouse secondary antibody. Slides were qualitatively scored under a microscope
+++, strong;++, good;+, weak; and − no reactivity
Pf rCSP-CAF09, P. falciparum recombinant circumsporozoite protein in adjuvant CAF09; P.b.-P.f. CSP-FL CD8CT, P. berghei–P. falciparum CSP full-length CD8 epitope C-terminus transgenic parasite