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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: J Cell Biochem. 2017 May 23;118(11):3834–3845. doi: 10.1002/jcb.26033

Figure 2. Orlistat and AICAR combination downregulates FA synthesis proteins.

Figure 2

PC3, DU145, and LNCaP cells were pre-treated with AICAR (1 mM) or compound C (5 μM) for 1 h. The cells were then either left untreated or were treated with Orlistat (100 μM) for 24 h. Cell lysates were collected and analyzed for FASN, mature SREBP-1c, pACC, and pAMPK protein expression by Western blotting. Blots were stripped and reprobed for ACC, AMPK, and β-actin protein expression as a loading control. Representative blots from three or more independent experiments are shown.