Figure 1. Fak floxed locus and rearrangement.

(a) Schematic of mouse Fak gene and targeted allele. Fak gene is composed of FERM, Kinase, PXXP, and FAT domains from N terminal to C terminal. Exon3 locating at FERM domain was flanked by LoxP sites. Primers used to identify the wild type (WT), floxed (Flox) and rearranged (FAK^R) alleles are shown as solid arrows: primer pair P1/P2 amplifies a 800-bp WT and 1000-bp Flox bands; primer pair P1/P3 amplifies a 550-bp rearranged floxed Fak allele (FAK^R). (b) Genomic DNA was extracted from mouse tail and analyzed by PCR using P1, P2, and P3 primers to distinguish different types of alleles with or without the rearrangement of Fak floxed allele.